Ames MPF Penta 2 - 10 Sample Kit for Ames Test with 5 Strains

Art. No.: B10-513-S2-P

Kit includes 5 x 480 Measuring Points for 10 Samples if tested in 5 strains, 3 Replicates, 6 Dilutions, +/- S9, Negative and Positive Controls.

5 Strains: TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA[pKM101]
PB/ß-Naphtoflavone-induced rat liver S9
Positive controls
Media

Not included in the kit: S9 cofactor solutions, microplates

Product Description
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Ames MPF Kit is a liquid microplate fluctuation assay kit, standardized and quality controlled. It is a ready to use Kit for the Ames mutagenicity assay and includes 10 vials each of semisolid TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA(pKM101), Booster, Growth -, Exposure -, Reversion Indicator Media, Positive Controls, Post-Mitochondrial Supernatant S9 from lyophilized PB/ß-Naphtoflavone-induced rat liver S9. The assay is using the same strains, positives controls and rat liver S9, cofactors as listed in OECD 471 and is quality controlled according to OECD 471. The liquid media used in this assay allow to test successfully pharmaceuticals, (agro-)chemicals, cosmetics, water, soil, many publication are referenced on this homepage. Especially volatile compounds, or genotoxic impurities available in low quantities, e.g. nitrosamines can be tested successfully with the Ames MPF.

Characteristics

Quality controlled kit including all strains, S9, media, positive controls - no inhouse quality control, no sterility control are necessary
Certificate of Analysis is provided
Excel Calculation Sheet to support data Interpretation free of charge
Compliant with ICH M7 allowing testing for very small quantities of genotoxic impurities, e.g. nitrosamines
Suitable for volatile test compounds
Number of tests corresponds to 5 x 480 agar plates.
Standardized procedure
Shipped at room temperature from external storage in Germany or from Switzerland
High sensitivity and excellent correlation with agar plate test (see referenced publications)
4 times less test compound as compared to the agar plate test, optimized for screening purposes
Less hands-on-time and simultaneous processing of several replicates
Automation available - high throughput
3Rs - 12 fold less consumption of rat liver liver S9, significant less test animals sacrificed
Less error prone due to easy colorimetric read-out
Waste - Significant less plastic waste and thus reduced contaminated waste in Environment
Technology transfer available, please contact info@xenometrix.ch

The Ames test is a bacterial mutation reversion assay which evaluates the mutagenic potential of chemical, pharmaceutical or cosmetic substances, food additives, food packaging material, mixtures, drinking-, waste water or soils. The bacterial strains carry several mutations that inactivate the gene involved in the synthesis of histidine (s.typhimurium) or tryptophan (E.coli), i.e. they grow only in a medium supplemented with either his or trp. Mutagens induce mutations which may restore the ability of the bacteria to re-synthesize the amino acid (his or trp) and to grow in medium without his or trp. Growth of bacteria induces a pH shift which is easily detected by the yellow color due to the pH-indicator included in the medium.

S9 buffer salts are not included in the kit and available separately: S9 Cofactor Kit (Art.No. PCO-0800)

Also available:

B01-513-S2-P Ames MPF Penta 2 with S9 from PB/NF induced lyophilized rat liver, 1 sample kit
B10-513 Ames MPF Penta 2, basic kit without S9 and positive controls, 10 sample kit
Individual products for Ames Test with Ames MPF, such as S9, media, positive controls, etc.
Service Analytics for Ames Tests with all techniques under GLP and non GLP conditions (OECD 471), also for testing very low sample quantities in "µg" or for mixtures with NanoAmesTM, ultra-miniaturized Ames Test (ICH M7, 35 µg/compound in 5 strains)
For Ames MPF Penta 1 kits, please contact info@xenometrix.ch