PB/Naphtoflavone induced rat liver S9 Fraction for Ames Test, MNvit, Chromosomal Aberration Test

Art. No.: PRS-PB01

1 ml, lyophilized

Product Description
Documents (5)

Due to bacteria being unable to metabolize molecules, the mutagenic potential of samples is assessed directly and in the presence of metabolic activation system: Microsomal fraction S9 of Phenobarbital/ß-Naphtoflavone induced rat liver homogenate. Rat liver S9 is used in the Ames Test (OECD 471), in vitro Micronucleus Test (MNvit, OECD 487) and in Chromosomal Aberration Test (OECD 473) for chemicals, xenobiotics, herbicides, pesticides, biocides, cosmetics, pharmaceutical preparations or soil, waste water.

Phenobarbital/ß-Naphtoflavone induced hepatic S9 after reconstitution is a buffered solution containing the post mitochondrial S9 fraction of a pool of livers from male SpragueDawley rats. Liver S9 fractions (the 9000g supernatant of a liver homogenate) contain both microsomal and cytosolic fractions. S9 fraction includes many cytosolic enzymes: N-acetyl transferases, methyltransferases, sulfotransferases, glutathione transferases, aldehyde oxidase, xanthine oxidase. Both Phase I (Cytochrome P450-Monooxigenases) and Phase II metabolites act simultaneously on test samples. Due to the fact that the enzymes in the S9 fractions are diluted significantly, cofactors such as e.g. β-Nicotinamide adenine dinucleotide phosphate-regenerating system (NADPH, Phase I oxidation) are required for activity. Cofactors are added to the S9 preparation before the experiment (S9 Cofactor Kit, art.no. PCO-0800).

Characteristics

High quality of lyophilized S9 with comparable activity to frozen rat liver S9 (see attachment below)
Protein Concentration: 25 - 35 mg/ml
Storage: -20 or -80 ^oC
Shipment: with Cool Blocks from external storage in Germany or from Switzerland
Shelf Life: 12 -36 months
S9 is quality controlled in all strains available from Xenometrix: TA98, TA100, TA1535, TA1537, E.coli uvrA(pKM101), E.coli Combo (E.coli uvrA combined with E.coli pKM101)^, please see Certificate of Analysis below
In full compliance with OECD 471 guideline, ICH M7, OECD 487, OECD 473, ISO13449.
Requested volumes in different Ames test: 0.44 mL/strain/compound in MicroAmes24, 0.35 mL/strain/ compound in Ames MPF, 1.4 mL / strain/ compound in Ames agar plate Test
Can also be used for micronucleus test (MNvit) and Chromosomal Aberration Test
Is also included in all in one Ames MPF kits

Also available

S9 Cofactor Kit, art.no. PCO-0800
Post-Mitochondrial Supernatant S9 Fraction from Aroclor 1254 induced rat liver, lyophilized, 1 mL and 2 mL*
Kits including S9:Ames MPF 98/100, Ames MPF Penta 2

*Please note that Aroclor 1254 is in conflict with Stockholm Convention on Persistent Organic Pollutants and has been banned. Therefore, the availability of the Aroclor 1254-induced S9 cannot be guaranteed in the future.