S9 Cofactor Kit for Metabolic Activation in Ames Test, Micronucleus Test

Art. No.: PCO-0800

20 ml BufferSalts, 0.9 ml G-6-P , 3.5 ml NADP Sufficient for 30 Samples per Strain in Ames MPF Test Kit Sufficient for 8 Samples per Strain in MicroAmes24 Sufficient for 150 samples per Strain in NanoAmes

Product Description
Documents (3)

S9 Cofactor Kit is a ready-to-use kit containing Buffer Salts, G-6-P and NADP for preparing the S9 mix in metabolic activation studies, especially for the Ames Test and Micronuclues Test.

Liver S9 fractions (the 9000g supernatant of a liver homogenate) contain both microsomal and cytosolic fractions. S9 fraction includes many cytosolic enzymes: N-acetyl transferases, methyltransferases, sulfotransferases, glutathione transferases, aldehyde oxidase, xanthine oxidase, thus both Phase I and II metabolites act simultaneously. Due to the fact that the enzymes in the S9 fractions are diluted significantly, cofactors such as e.g. β-Nicotinamide adenine dinucleotide phosphate-regenerating system (NADPH, Phase I oxidation) are required for activity.
Microsomes do not include the cytosolic enzymes, they include the endoplasmic reticulum subcellular fraction only with cytochrome P450, CYPs, Uridine 5'-diphospho-glucuronosyltransferase (UGT)).

Characteristics

This kit is quality controlled for the use with the miniaturized Ames MPF, Ames II Test kits, MicroAmes24, NanoAmes^TM.
In full compliance with OECD 471 guideline.
Sufficient for all 1 sample Ames MPF kits, all 10 sample 1- and 2- strain kits, 2 Cofactor kits are needed for the 10 sample PENTA 1 and 2 kits.
Storage of S9 Buffer Salts: 2-8^oC, S9 G-6-P: -20^oC, S9 NADP: -20^oC
The products are shipped from external warehouse in Germany

Also available

Post-Mitochondrial Supernatant S9 Fraction from PB/ß-Naphtoflavone induced rat liver, lyophilized, 1 mL and 2 m
Post-Mitochondrial Supernatant S9 Fraction from Aroclor 1254 induced rat liver, lyophilized, 1 mL and 2 mL*

*Aroclor 1254 is in conflict with Stokholm Convention on Persistent Organic Pollutants and has been banned. Therefore, the availability of the Aroclor 1254-induced S9 cannot be guaranteed in the future.

PCO-0800 - S9 Cofactor Kit

MSDS

PCO-0800 - S9 Cofactor Kit

Comparison of S9 Preparations

Leaflet

Comparison of S9 Preparations

Service Analytics Genotoxicity

Service Analytics Genotoxicity